Direct Incorporation of Glucosamine and N-Acetylglucosamine into Exopolymers by Gluconacetobacter xylinus (5Acetobacter xylinum) ATCC 10245: Production of Chitosan-Cellulose and Chitin-Cellulose Exopolymers
نویسندگان
چکیده
Gluconacetobacter xylinus (5Acetobacter xylinum) ATCC 10245 incorporated 2-amino-2-deoxy-D-glucose (glucosamine) and 2-acetamido-2-deoxy-D-glucose (N-acetylglucosamine), but not 3-O-methyl-D-glucose or 2-deoxyD-glucose into exopolymers. Incorporation was confirmed by gas chromatography with and without mass spectrometry, Fourier transform infrared, and H nuclear magnetic resonance. The average molar percentage of glucosamine and N-acetylglucosamine in the exopolymers was about 18%.
منابع مشابه
The synthesis of cellulose in cell-free extracts of Acetobacter xylinum.
In a previous publication (2) evidence was presented that the synthesis of chitin in Neurospora crassa occurs by way of a glycosyl transfer from uridine diphosphate-N-acetylglucosamine. The similarity of the structure of chitin and cellulose suggested that a like mechanism may be operative in the biosynthesis of cellulose. It has been shown by Hestrin and Schramm (3) that non-viable lyophilized...
متن کاملN-acetylglucosamine 6-Phosphate Deacetylase (nagA) Is Required for N-acetyl Glucosamine Assimilation in Gluconacetobacter xylinus
Metabolic pathways for amino sugars (N-acetylglucosamine; GlcNAc and glucosamine; Gln) are essential and remain largely conserved in all three kingdoms of life, i.e., microbes, plants and animals. Upon uptake, in the cytoplasm these amino sugars undergo phosphorylation by phosphokinases and subsequently deacetylation by the enzyme N-acetylglucosamine 6-phosphate deacetylase (nagA) to yield gluc...
متن کاملBiotechnological Production of Cellulose by Gluconacetobacter Xylinus from Agricultural Waste
The purpose of this study was to utilize low quality date syrup, a rich and available source of nutrient in Iran, for the production of bacterial cellulose using Gluconacetobacter xylinus. Static batch fermentationfor the purpose of cellulose production by G. xylinus (PTCC, 1734) was studied using low quality date syrupand sucrose solution (Bx. 10%) as fermentation media at 28°C. Re...
متن کاملProduction of bacterial cellulose from alternate feedstocks.
Production of bacterial cellulose by Acetobacter xylinum ATCC 10821 and 23770 in static cultures was tested from unamended food process effluents. Effluents included low-solids (LS) and high-solids (HS) potato effluents, cheese whey permeate (CW), or sugar beet raffinate (CSB). Strain 23770 produced 10% less cellulose from glucose than did strain 10821 and diverted more glucose to gluconate. Un...
متن کاملTransgenic expression of Gluconacetobacter xylinus strain ATCC 53582 cellulose synthase genes in the cyanobacterium Synechococcus leopoliensis strain UTCC 100
We report the transfer of cellulose synthesis genes (acsABDC) from the heterotropic alpha proteobacterium, Gluconacetobacter xylinus strain ATCC 53582 to a photosynthetic microbe (Synechococcus leopoliensis strain UTCC 100). These genes were functionally expressed in this cyanobacterium, resulting in the production of non-crystalline cellulose. Although the cellulose lacks the structural integr...
متن کامل